Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of FMOD Antibody in mouse cerebellum tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with FMOD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | FMOD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fibromodulin, FM, Collagen-binding 59 kDa protein, Keratan sulfate proteoglycan fibromodulin, KSPG fibromodulin, FMOD, FM, SLRR2E |
| Product description | Fibromodulin is a member of a family of small interstitial proteoglycans, containing a central region composed of leucine-rich repeats with 4 keratan sulfate chains flanked by disulfide-bonded terminal domains. It may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix.1) Ehret,G.B., et.al, Eur. J. Hum. Genet. 17 (12), 1650-1657 (2009) |
| Immunogen | This FMOD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-364 amino acids from the C-terminal region of human FMOD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FMOD |
|---|---|
| Alternative Names | Fibromodulin FM Collagen-binding 59 kDa protein Keratan sulfate proteoglycan fibromodulin KSPG fibromodulin FMOD FM SLRR2E |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of FMOD Antibody in mouse cerebellum tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with FMOD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
