Application
Immunohistochemistry of Fn14 in human liver tissue with Fn14 antibody at 2.5 μg/mL.Application
Western blot analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of Fn14 in Human Liver cells with Fn14 antibody at 10 μg/mL.IF
C, Immunofluorescence for Fn14 (green) and DAPI (blue) demonstrating increased Fn14 levels after TWEAK (100 ng/mL) treatment for 24 h; Fn14 siRNA inhibition attenuated the effect in HL-1 atrial myocytes.| Product Name | Fn14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Fn14 Antibody: TWEAK is a TNF family member produced by IFN-gamma-stimulated monocytes and induces multiple cell death pathways including caspase-dependent apoptosis and cathepsin B-dependent necrosis. These pathways are mediated by the binding of TWEAK by fibroblast growth factor 14 (Fn14), a member of the TNF receptor superfamily. Yeast two-hybrid experiments have shown that FN14 will bind to the TNFR-associated factors TRAF1, TRAF2, TRAF3, and TRAF5, suggesting that the signaling pathway induced by TWEAK may be mediated by one or more of these adaptor molecules. Fn14 is induced during liver regeneration and is highly expressed in hepatocellular carcinomas. In addition, TWEAK induces liver progenitor cell proliferation, suggesting Fn14 may play a role in hepatocyte growth control and liver neoplasia.1) Chicheportiche Y, Bourdon PR, Xu H, et al. TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J. Biol. Chem.1997; 272:32401-10. |
| Immunogen | Fn14 antibody was raised against a human IgG1 Fc-tagged Fn14 protein. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:500 IF:1:100~1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF12A |
|---|---|
| Alternative Names | Fn14 Antibody: FN14 CD266 TWEAKR FN14 Tumor necrosis factor receptor superfamily member 12A Fibroblast growth factor-inducible immediate-early response protein 14 FGF-inducible 14 |
| Sequence Similarities | 3) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of Fn14 in human liver tissue with Fn14 antibody at 2.5 μg/mL.
Application
Western blot analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of Fn14 in Human Liver cells with Fn14 antibody at 10 μg/mL.
IF
C, Immunofluorescence for Fn14 (green) and DAPI (blue) demonstrating increased Fn14 levels after TWEAK (100 ng/mL) treatment for 24 h; Fn14 siRNA inhibition attenuated the effect in HL-1 atrial myocytes.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:500 IF:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Fn14 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Fn14 Antibody is supplied in PBS containing 0.02% sodium azide. |
