Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from COS7 cells (Lane 2), using FOS (Phospho-Thr232) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | FOS (phospho Thr232) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOS, G0S7, Cellular oncogene fos |
| Product description | Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.1) van Straaten F. Proc. Natl. Acad. Sci. U.S.A. 80:3183-3187(1983). |
| Immunogen | FOS (phospho Thr232) antibody was raised against a peptide sequence around phosphorylation site of threonine 232 (V-A-T (p)-P-E) derived from Human FOS . |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOS |
|---|---|
| Alternative Names | FOS G0S7 Cellular oncogene fos |
| Molecular Weight(MW) | 48 kDa |
| Tissue Specificity | The antibody detects endogenous levels of FOS only when phosphorylated at threonine 232. |
| Entrez Gene | 2353 |
|---|---|
| Protein Accession | NP_005243.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from COS7 cells (Lane 2), using FOS (Phospho-Thr232) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM279014