Application
Fluorescent confocal image of A549 cell stained with FOSL2 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOSL2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhalloidiApplication
Western blot analysis in U251 cell line lysates (35ug/lane).| Product Name | FOSL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fos-related antigen 2, FRA-2, FOSL2, FRA2 |
| Product description | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.1) Bozec, A., et al. J. Cell Biol. 190(6):1093-1106(2010) |
| Immunogen | This FOSL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-248 amino acids from the C-terminal region of human FOSL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOSL2 |
|---|---|
| Alternative Names | Fos-related antigen 2 FRA-2 FOSL2 FRA2 |
| Molecular Weight(MW) | 35 kDa |
Application
Fluorescent confocal image of A549 cell stained with FOSL2 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOSL2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidi
Application
Western blot analysis in U251 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
