Application
Western blot analysis of FOXA1 in 293 cell lysate with FOXA1 antibody at 1 μg/mL| Product Name | FOXA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXA1 Antibody: HNF3A, TCF3A, HNF3A, Hepatocyte nuclear factor 3-alpha, Forkhead box protein A1, HNF-3-alpha |
| Product description | FOXA1 Antibody: FOXA1 is one of three members of the FOXA family, a subset of the forkhead family of transcription factors which play vital roles in development. It has also been implicated in the development of a number of other organs including the androgen and estrogen regulated tissues of the breast and prostate. FOXA1 modulates the transcriptional activity of nuclear hormone receptors and regulates apoptosis by inhibiting the expression of BCL-2. It is an essential protein for the transcriptional activity of both androgen receptor (AR) and estrogen receptor-α (ER). FOXA1 plays a pivotal role from early stage cancer through to drug resistant and metastatic disease. FOXA1 is not only an attractive therapeutic target but could potentially function as a novel biomarker.1) Hannenhalli S and Kaestner KH. The evolution of Fox genes and their role in development and disease. Nat. Rev. Genet. 2009; 10:233-40. |
| Immunogen | Rabbit polyclonal FOXA1 antibody was raised against a 17 amino acid peptide near the center of human FOXA1. The immunogen is located within amino acids 270 - 320 of FOXA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| FOXA1 antibody can be used for detection of FOXA1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXA1 |
|---|---|
| Alternative Names | FOXA1 Antibody: HNF3A TCF3A HNF3A Hepatocyte nuclear factor 3-alpha Forkhead box protein A1 HNF-3-alpha |
| Molecular Weight(MW) | 52 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (86%) |
| Tissue Specificity | Multiple isoforms of FOXA1 exists as a result of alternative splicing event. |
Application
Western blot analysis of FOXA1 in 293 cell lysate with FOXA1 antibody at 1 μg/mL| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | FOXA1 antibody can be used for detection of FOXA1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FOXA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | FOXA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
