Application
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in A2058 cell line lysates (15ug/lane).Application
immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the for immunohistochemistry.| Product Name | FOXC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, MFH-1 protein, Transcription factor FKH-14, FOXC2, FKHL14, MFH1 |
| Product description | FOXC2 belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.1) van Dongen, M.J., et al. J. Mol. Biol. 296(2):351-359(2000) |
| Immunogen | This FOXC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the Central region of human FOXC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXC2 |
|---|---|
| Alternative Names | Forkhead box protein C2 Forkhead-related protein FKHL14 Mesenchyme fork head protein 1 MFH-1 protein Transcription factor FKH-14 FOXC2 FKHL14 MFH1 |
| Molecular Weight(MW) | 54 kDa |
Application
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in A2058 cell line lysates (15ug/lane).
Application
immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the for immunohistochemistry.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
