Foxd1 is transcription factor required for formation of positional identity in the developing retina, regionalization of the optic chiasm and morphogenesis of the kidney. Mice lacking Foxd1 show disrupted cell identity in the ventrotemporal area of the retina and aberrant morphogenesis of the optic chiasm. Their kidneys remain fused, have a disorganised ureteric tree and fail to ascend to a lumbar position.1) Airik, R., et al., (2006) J. Clin. Invest. 116 (3), 663-674.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse FOXD1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
FOXD1 antibody can be used for detection of FOXD1 by ELISA at 1:312500. FOXD1 antibody can be used for detection of FOXD1 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse SP2/0 at 0.0625 ug/ml.
Positive Control
1) SP2/0 Cell Lysate
Application Notes
FOXD1 antibody can be used for detection of FOXD1 by ELISA at 1:312500. FOXD1 antibody can be used for detection of FOXD1 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store FOXD1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.