Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).Application
Fluorescent image of Hela cell stained with FOXN3 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7unitsApplication
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | FOXN3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Forkhead box protein N3, Checkpoint suppressor 1, FOXN3, C14orf116, CHES1 |
| Product description | This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in distinct isoforms. [provided by RefSeq].1) Samaan, G., et al. Biochem. Biophys. Res. Commun. 400(1):60-65(2010) |
| Immunogen | This FOXN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human FOXN3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXN3 |
|---|---|
| Alternative Names | Forkhead box protein N3 Checkpoint suppressor 1 FOXN3 C14orf116 CHES1 |
| Molecular Weight(MW) | 54 kDa |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application
Fluorescent image of Hela cell stained with FOXN3 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units
Application
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
