Application
Immunocytochemistry of FOXO3 in A-20 cells with FOXO3 antibody at 4 μg/mL.Application
Western blot analysis of FOXO3 in A-20 cell lysate with FOXO3 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | FOXO3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXO3 Antibody: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, Forkhead box protein O3, AF6q21 protein |
| Product description | FOXO3 Antibody: FOXO3 is a ubiquitously expressed 75 kDa protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. FOXO3 is involved with mTOR in the regulation of autophagy in skeletal muscle, and activates protein degradation in atrophying muscle cells. FOXO3 has also been implicated in several neurodegenerative disorders including aging, neuromuscular disease, systemic lupus erythmatosus, stroke and diabetic complications.1) Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99. |
| Immunogen | FOXO3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human FOXO3. The immunogen is located within amino acids 40 - 90 of FOXO3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| FOXO3 antibody can be used for detection of FOXO3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXO3 |
|---|---|
| Alternative Names | FOXO3 Antibody: FOXO2 AF6q21 FKHRL1 FOXO3A FKHRL1P2 Forkhead box protein O3 AF6q21 protein |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
Application
Immunocytochemistry of FOXO3 in A-20 cells with FOXO3 antibody at 4 μg/mL.
Application
Western blot analysis of FOXO3 in A-20 cell lysate with FOXO3 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | FOXO3 antibody can be used for detection of FOXO3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FOXO3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FOXO3 Antibody is supplied in PBS containing 0.02% sodium azide. |
