FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.1) Submitted (17-AUG-2004) MIPS, Ingolstaedter Landstr.1, D-85764 Neuherberg, GERMANY.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FOXO3A.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
FOXO3A antibody can be used for detection of FOXO3A by ELISA at 1:62500. FOXO3A antibody can be used for detection of FOXO3A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Positive Control
1) MU Lysate
Application Notes
FOXO3A antibody can be used for detection of FOXO3A by ELISA at 1:62500. FOXO3A antibody can be used for detection of FOXO3A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store FOXO3A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.