Application
Antibody used in IHC on Mouse spleen at 1:100.Application
Antibody used in IHC on Human Liver.Application
Antibody used in WB on Human HepG2 at 5.0-8.0 ug/ml.IHC
Positive staining of 15-PGDH protein was mainly found in the cytoplasm of gastric cancer (GC) tumor cells: (A) High/moderately differentiated GC, positive staining; (B) Poorly differentiated GC, negative staining. IHC staining of FOXP3 expression was mainly observed in the cytoplasm of lymphocytes in gastric cancer tissues: FOXP3 expression in high/moderately differentiated GC was lower (C) than in poorly differentiated GC (D) tissues. (×200).| Product Name | FOXP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Forkhead box protein P3 (FOXP3, Scurfin, Zinc finger protein JM2) encodes a novel member of the forkhead family of transcription factors. It presumably represses transcription, playing a paramount role in determining the amplitude of the response of CD4 T cells to activation1) Takahata, Y., et al., (2004) Exp.Hematol.32(7), 622-629. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FOXP3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| FOXP3 antibody can be used for detection of FOXP3 by ELISA at 1:312500. FOXP3 antibody can be used for detection of FOXP3 by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | FOXP3 |
|---|---|
| Alternative Names | FOXP3 JM2 AIID IPEX PIDX XPID DIETER |
| Molecular Weight(MW) | 47 kDa |
Application
Antibody used in IHC on Mouse spleen at 1:100.
Application
Antibody used in IHC on Human Liver.
Application
Antibody used in WB on Human HepG2 at 5.0-8.0 ug/ml.
IHC
Positive staining of 15-PGDH protein was mainly found in the cytoplasm of gastric cancer (GC) tumor cells: (A) High/moderately differentiated GC, positive staining; (B) Poorly differentiated GC, negative staining. IHC staining of FOXP3 expression was mainly observed in the cytoplasm of lymphocytes in gastric cancer tissues: FOXP3 expression in high/moderately differentiated GC was lower (C) than in poorly differentiated GC (D) tissues. (×200).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | FOXP3 antibody can be used for detection of FOXP3 by ELISA at 1:312500. FOXP3 antibody can be used for detection of FOXP3 by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FOXP3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 200 μL of distilled water. Final antibody concentration is 1 mg/mL. |
