Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with FPRL2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of FPRL2 Antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | FPRL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | N-formyl peptide receptor 3, FMLP-related receptor II, FMLP-R-II, Formyl peptide receptor-like 2, FPR3, FPRH1, FPRL2 |
| Product description | Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.1) Yang,D., et.al., J. Leukoc. Biol. 72 (3), 598-607 (2002) |
| Immunogen | This FPRL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-333 amino acids from the Central region of human FPRL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FPR3 |
|---|---|
| Alternative Names | N-formyl peptide receptor 3 FMLP-related receptor II FMLP-R-II Formyl peptide receptor-like 2 FPR3 FPRH1 FPRL2 |
| Molecular Weight(MW) | 40 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with FPRL2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of FPRL2 Antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
