Application
Confocal immunofluorescent analysis of Fructose 6 Phosphate Kinase (PFKM) Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PFKM polyclonal antibody in Hela cell line lysate| Product Name | Fructose 6 Phosphate Kinase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-M, 27111 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, PFK-A, Phosphohex |
| Product description | Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.1) Howard, T.D., et al., Genomics 34(1):122-127 (1996). |
| Immunogen | This Fructose 6 Phosphate Kinase (PFKM) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 746-776 amino acids from the C-terminal region of human Fructose 6 Phosphate Kinase (PFKM). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PFKM |
|---|---|
| Alternative Names | ATP-dependent 6-phosphofructokinase muscle type {ECO:0000255|HAMAP-Rule:MF_03184} ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184} PFK-M 27111 {ECO:0000255|HAMAP-Rule:MF_03184} 6-phosphofructokinase type A Phosphofructo-1-kinase isozyme A PFK-A Phosphohex |
| Molecular Weight(MW) | 85 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Confocal immunofluorescent analysis of Fructose 6 Phosphate Kinase (PFKM) Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PFKM polyclonal antibody in Hela cell line lysate| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
