Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).Application
FUT4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | FUT4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-(1,3)-fucosyltransferase 4, 241-, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, FUT4, ELFT, FCT3A |
| Product description | The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).1) Yang, X.S., et al. J. Cell. Physiol. 225(2):612-619(2010) Trubiani, O., et al. J. Cell. Physiol. 225(1):123-131(2010) Pruszak, J., et al. Stem Cells 27(12):2928-2940(2009) Ogata, K., et al. Haematologica 94(8):1066-1074(2009) Read, T.A., et al. Cancer Cell 15(2):135-147(2009) |
| Immunogen | This FUT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-524 amino acids from the C-terminal region of human FUT4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | FUT4 |
|---|---|
| Alternative Names | Alpha-(1 3)-fucosyltransferase 4 241- ELAM-1 ligand fucosyltransferase Fucosyltransferase 4 Fucosyltransferase IV Fuc-TIV FucT-IV Galactoside 3-L-fucosyltransferase FUT4 ELFT FCT3A |
| Molecular Weight(MW) | 59 kDa |
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Application
FUT4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
