Application
Western blot analysis of lysate from Hela cell line, using FUT8 Antibody at 1:1000 at each lane.Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).Application
FUT8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | FUT8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-(1,6)-fucosyltransferase, Alpha1-6FucT, Fucosyltransferase 8, GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase, GDP-fucose--glycoprotein fucosyltransferase, Glycoprotein 6-alpha-L-fucosyltransferase, FUT8 |
| Product description | This enzyme belongs to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternatively spliced variants encoding different isoforms have been identified.1) Rose, J. Phd, et al. Mol. Med. (2010) In press : |
| Immunogen | This FUT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-357 amino acids from the Central region of human FUT8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FUT8 |
|---|---|
| Alternative Names | Alpha-(1 6)-fucosyltransferase Alpha1-6FucT Fucosyltransferase 8 GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1 6-fucosyltransferase GDP-fucose--glycoprotein fucosyltransferase Glycoprotein 6-alpha-L-fucosyltransferase FUT8 |
| Molecular Weight(MW) | 67 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of lysate from Hela cell line, using FUT8 Antibody at 1:1000 at each lane.
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
FUT8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
