Application
Antibody used in WB on Human Placenta at 0.625 ug/ml.Application
Antibody used in WB on Human 293T at: 1:1000 (Lane 1: 10ug hFXYD5 transfected 293T lysate, Lane 2: 10ug 293T lysate).| Product Name | FXYD5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FXYD5, RIC, IWU1, KCT1, OIT2, DYSAD, HSPC113, PRO6241 |
| Product description | FXYD5 is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIon Channel (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIon Channel) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, has not been characterized as a protein.1) Batistatou, A., et al., (2005) Br. J. Cancer 93 (12), 1382-1387. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FXYD5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| FXYD5 antibody can be used for detection of FXYD5 by ELISA at 1:312500. FXYD5 antibody can be used for detection of FXYD5 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FXYD5 |
|---|---|
| Alternative Names | FXYD5 RIC IWU1 KCT1 OIT2 DYSAD HSPC113 PRO6241 |
| Molecular Weight(MW) | 20 kDa, 19 kDa |
Application
Antibody used in WB on Human Placenta at 0.625 ug/ml.
Application
Antibody used in WB on Human 293T at: 1:1000 (Lane 1: 10ug hFXYD5 transfected 293T lysate, Lane 2: 10ug 293T lysate).| Positive Control | 1) Cat. No. 1309 - Human Placenta Lysate |
|---|---|
| Application Notes | FXYD5 antibody can be used for detection of FXYD5 by ELISA at 1:312500. FXYD5 antibody can be used for detection of FXYD5 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FXYD5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
