Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of FYN using rabbit polyclonal FYN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FYN gene.Application
Western blot analysis of anti-FYN Pab in mouse liver tissue lysate| Product Name | FYN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tyrosine-protein kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, SLK, p59-Fyn, FYN |
| Product description | FYN is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein.1) Taniguchi, S., et al., Biochem. Biophys. Res. Commun. 306(1):151-155 (2003). Chan, B., et al., Nat. Cell Biol. 5(2):155-160 (2003). Goldsmith, J.F., et al., Biochem. Biophys. Res. Commun. 298(4):501-504 (2002). Freund, C., et al., EMBO J. 21(22):5985-5995 (2002). Parravicini, V., et al., Nat. Immunol. 3(8):741-748 (2002). |
| Immunogen | This FYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-43 amino acids from the N-terminal region of human FYN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FYN |
|---|---|
| Alternative Names | Tyrosine-protein kinase Fyn Proto-oncogene Syn Proto-oncogene c-Fyn Src-like kinase SLK p59-Fyn FYN |
| Molecular Weight(MW) | 61 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of FYN using rabbit polyclonal FYN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FYN gene.
Application
Western blot analysis of anti-FYN Pab in mouse liver tissue lysate| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
