Application
Antibody used in IHC on human kidney at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | FZD7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FZD7, FzE3 |
| Product description | FZD7 is 7-transmembrane domain protein that is receptor for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif.Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.1) Vincan, E., (2005) Differentiation 73 (4), 142-153. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FZD7. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| FZD7 antibody can be used for detection of FZD7 by ELISA at 1:1562500. FZD7 antibody can be used for detection of FZD7 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FZD7 |
|---|---|
| Alternative Names | FZD7 FzE3 |
| Molecular Weight(MW) | 63 kDa |
Application
Antibody used in IHC on human kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | FZD7 antibody can be used for detection of FZD7 by ELISA at 1:1562500. FZD7 antibody can be used for detection of FZD7 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FZD7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
