The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V (D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.1) Sawchuk, D.J., et al., (2004) J. Biol. Chem. 279 (28), 29821-29831.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human G22P1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
G22P1 antibody can be used for detection of G22P1 by ELISA at 1:312500. G22P1 antibody can be used for detection of G22P1 by western blot at 0.6 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
G22P1 antibody can be used for detection of G22P1 by ELISA at 1:312500. G22P1 antibody can be used for detection of G22P1 by western blot at 0.6 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store G22P1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt