Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of G6PD Antibody in K562,MCF-7 cell line lysates (35ug/lane)Application
G6PD Antibody IHC analysis in formalin fixed and paraffin embedded testis followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | G6PD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glucose-6-phosphate 1-dehydrogenase, G6PD, G6PD |
| Product description | G6PD encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia.1) Louicharoen, C., et al. Science 326(5959):1546-1549(2009) |
| Immunogen | This G6PD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the Central region of human G6PD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | G6PD |
|---|---|
| Alternative Names | Glucose-6-phosphate 1-dehydrogenase G6PD G6PD |
| Molecular Weight(MW) | 59 kDa |
Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of G6PD Antibody in K562,MCF-7 cell line lysates (35ug/lane)
Application
G6PD Antibody IHC analysis in formalin fixed and paraffin embedded testis followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
