Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Gab1 (Phospho-Tyr627).Application
Immunofluorescence staining of methanol-fixed HeLa cells using Gab1(Phospho-Tyr627).| Product Name | Gab1 (phospho Tyr627) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADDA, Growth factor receptor bound protein 2-associated protein 1 |
| Product description | The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.1) Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904. Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307. Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773. |
| Immunogen | Gab1 (Phospho-Tyr627) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y (p) -L-D) derived from Human Gab1. |
| Modification | p-Tyr627 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAB1 |
|---|---|
| Alternative Names | ADDA Growth factor receptor bound protein 2-associated protein 1 |
| Molecular Weight(MW) | 110 kDa |
| Tissue Specificity | This antibody detects endogenous level of Gab1 only when phosphorylated at tyrosine 627. |
| Entrez Gene | 2549 |
|---|---|
| Protein Accession | NP_002030.2 |
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Gab1 (Phospho-Tyr627).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Gab1(Phospho-Tyr627).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
