Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Gab2 (Phospho-Tyr643) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 3T3 cells (Lane 2), using Gab2 (Phospho-Tyr643) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | Gab2 (phospho Tyr643) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | pp100, GABJ, KIAA0571 |
| Product description | This gene is a member of the GRB2-associated binding protein (GAB) gene family and is similar to the GAB1 gene. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.1) Nishida K., Blood 93:1809-1816(1999). |
| Immunogen | Gab2 (phospho Tyr643) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 643 (V-D-Y (p)-V-Q) derived from Human Gab2 . |
| Modification | p-Tyr643 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAB2 |
|---|---|
| Alternative Names | pp100 GABJ KIAA0571 |
| Molecular Weight(MW) | 74 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Gab2 only when phosphorylated at tyrosine 643. |
| Entrez Gene | 9846 |
|---|---|
| Protein Accession | NP_536739.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Gab2 (Phospho-Tyr643) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 3T3 cells (Lane 2), using Gab2 (Phospho-Tyr643) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
