Application
Immunofluorescence of GABARAPL2 in mouse brain tissue with GABARAPL2 Antibodyat 20 μg/mL.Application
Immunohistochemistry of GABARAPL2 in rat brain tissue with GABARAPL2 antibody at 5 μg/mL.Application
Immunofluorescence of GABARAPL2 in rat brain tissue with GABARAPL2 antibody at 20 μg/mL.Application
Immunohistochemistry of GABARAPL2 in mouse brain tissue with GABARAPL2 Antibodyat 5 μg/mL.Application
Western blot analysis of GABARAPL2 in human brain tissue lysate with GABARAPL2 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | GABARAPL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GABARAPL2 Antibody: ATG8, GEF2, ATG8C, GEF-2, GATE16, GATE-16, FLC3A, Gamma-aminobutyric acid receptor-associated protein-like 2, Ganglioside expression factor 2 |
| Product description | Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system (1). It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC (2). GABARAPL2 (GABAA receptor-associated protein-like 2), also known as GATE16, was initially identified as a membrane transport modulator and is a mammalian ortholog to the autophagy protein ATG8 (3,4). It is thought that GABARAPL2 and other members of the ATG8 family act as scaffolds for assembly of the Unc-51 like kinase (ULK) complex in the formation of autophagosomes (5).1) Cherubini E, Gaiarsa JL, and Ben-Ari Y. GABA: an excitatory transmitter in early postnatal life. Trends Neurosci.1991; 14:515-19. |
| Immunogen | GABARAPL2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human GABARAPL2. The immunogen is located within the last 50 amino acids of GABARAPL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GABARAPL2 antibody can be used for detection of GABARAPL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GABARAPL2 |
|---|---|
| Alternative Names | GABARAPL2 Antibody: ATG8 GEF2 ATG8C GEF-2 GATE16 GATE-16 FLC3A Gamma-aminobutyric acid receptor-associated protein-like 2 Ganglioside expression factor 2 |
| Molecular Weight(MW) | Predicted: 13 kDa Observed: 13 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | GABARAPL2 antibody is human, mouse and rat reactive. Multiple isoforms of GABARAPL2 are known to exist. |
Application
Immunofluorescence of GABARAPL2 in mouse brain tissue with GABARAPL2 Antibodyat 20 μg/mL.
Application
Immunohistochemistry of GABARAPL2 in rat brain tissue with GABARAPL2 antibody at 5 μg/mL.
Application
Immunofluorescence of GABARAPL2 in rat brain tissue with GABARAPL2 antibody at 20 μg/mL.
Application
Immunohistochemistry of GABARAPL2 in mouse brain tissue with GABARAPL2 Antibodyat 5 μg/mL.
Application
Western blot analysis of GABARAPL2 in human brain tissue lysate with GABARAPL2 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | GABARAPL2 antibody can be used for detection of GABARAPL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GABARAPL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | GABARAPL2 antibody is supplied in PBS containing 0.02% sodium azide. |
