GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Netzer, C., (2008) Am. J. Med. Genet. B Neuropsychiatr. Genet. 147 (1), 37-41.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GABRA5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISA,WB
GABRA5 antibody can be used for detection of GABRA5 by ELISA at 1:312500. GABRA5 antibody can be used for detection of GABRA5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Heart at 0.2-1 ug/ml.
Application Notes
GABRA5 antibody can be used for detection of GABRA5 by ELISA at 1:312500. GABRA5 antibody can be used for detection of GABRA5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GABRA5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.