Application
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate bApplication
To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hGalectin-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Galectin-3 (38-171) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-3.| Product Name | Galectin-3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Galectin-3. Human Galectin-3 specific antibody was purified by affinity chromatography employing immobilized Galectin-3 matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,IHC,WB |
| Sandwich ELISA: To detect Galectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3. Western Blot: To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | LGALS3 |
|---|---|
| Alternative Names | L31 GAL3 MAC2 CBP35 GALBP GALIG LGALS2 Galectin-3 35 kDa lectin Gal-3 |
Application
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate b
Application
To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hGalectin-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Galectin-3 (38-171) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-3.| Application Notes | Sandwich ELISA: To detect Galectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3. Western Blot: To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Galectin-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
