Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with GALT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of MDA-MB468 cells using GALT Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of GALT Antibody in MDA-MB231 cell line lysates (35ug/lane)| Product Name | GALT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase, GALT |
| Product description | Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet.1) Leslie,N.D., et.al., Genomics 14 (2), 474-480 (1992) |
| Immunogen | This GALT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-373 amino acids from the C-terminal region of human GALT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GALT |
|---|---|
| Alternative Names | Galactose-1-phosphate uridylyltransferase Gal-1-P uridylyltransferase UDP-glucose--hexose-1-phosphate uridylyltransferase GALT |
| Molecular Weight(MW) | 43 kDa |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with GALT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of MDA-MB468 cells using GALT Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of GALT Antibody in MDA-MB231 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
