Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with GAS7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of GAS7 antibody in A2058 cell line lysates (35ug/lane)Application
Western blot analysis of GAS7 antibody in mouse cerebellum tissue lysates (35ug/lane)Application
Western Blot at 1:1000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.| Product Name | GAS7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development.1) Chao,C.C., J. Neurosci. Res. 81 (2), 153-162 (2005) |
| Immunogen | This GAS7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-465 amino acids from the C-terminal region of human GAS7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACS,IHC-P,WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | GAS7 |
|---|---|
| Alternative Names | Growth arrest-specific protein 7 GAS-7 GAS7 KIAA0394 |
| Molecular Weight(MW) | 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with GAS7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of GAS7 antibody in A2058 cell line lysates (35ug/lane)
Application
Western blot analysis of GAS7 antibody in mouse cerebellum tissue lysates (35ug/lane)
Application
Western Blot at 1:1000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM279534