Application
Western blot analysis of GATA2 using rabbit polyclonal GATA2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GATA2 gene.Application
Western blot analysis of GATA2 Antibody in K562 cell line lysates (35ug/lane)Application
Flow cytometric analysis of K562 cells using GATA2 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GATA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Endothelial transcription factor GATA-2, GATA-binding protein 2, GATA2 |
| Product description | GATA2 is a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. This protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages.1) Pan,X., et.al., J. Biochem. 127 (1), 105-112 (2000) |
| Immunogen | This GATA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-407 amino acids from the C-terminal region of human GATA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GATA2 |
|---|---|
| Alternative Names | Endothelial transcription factor GATA-2 GATA-binding protein 2 GATA2 |
| Molecular Weight(MW) | 51 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western blot analysis of GATA2 using rabbit polyclonal GATA2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GATA2 gene.
Application
Western blot analysis of GATA2 Antibody in K562 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of K562 cells using GATA2 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
