GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). GBP2 is a GTPase that converts GTP to GDP and GMP. Interferons are cytokines that have antiviral effects and inhibit tumor cell proliferation. They induce a large number of genes in their target cells, including those coding for the guanylate-binding proteins (GBPs). GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). The protein encoded by this gene is a GTPase that converts GTP to GDP and GMP.1) Lukasiewicz, R., (2007) J. Biol. Chem. 282 (32), 23036-23043.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GBP2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
GBP2 antibody can be used for detection of GBP2 by ELISA at 1:312500. GBP2 antibody can be used for detection of GBP2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
GBP2 antibody can be used for detection of GBP2 by ELISA at 1:312500. GBP2 antibody can be used for detection of GBP2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GBP2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.