Application
GCDFP-15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).Application
Confocal immunofluorescent analysis of GCDFP-15 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GCDFP-15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.1) Bryborn, M., et al. Allergy 65(2):220-228(2010) |
| Immunogen | This GCDFP-15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the C-terminal region of human GCDFP-15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIP |
|---|---|
| Alternative Names | Prolactin-inducible protein Gross cystic disease fluid protein 15 GCDFP-15 Prolactin-induced protein Secretory actin-binding protein SABP gp17 PIP GCDFP15 GPIP4 |
| Molecular Weight(MW) | 17 kDa |
Application
GCDFP-15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
Confocal immunofluorescent analysis of GCDFP-15 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
