Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.Application
Antibody used in IHC on Human Muscle.| Product Name | GCDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GCDH belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits.The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.1) Kimura, K., (2006) Genome Res. 16 (1), 55-65. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GCDH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| GCDH antibody can be used for detection of GCDH by ELISA at 1:1562500. GCDH antibody can be used for detection of GCDH by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GCDH |
|---|---|
| Alternative Names | GCDH ACAD5 GCD |
| Molecular Weight(MW) | 48 kDa |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Application
Antibody used in IHC on Human Muscle.| Application Notes | GCDH antibody can be used for detection of GCDH by ELISA at 1:1562500. GCDH antibody can be used for detection of GCDH by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GCDH antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
