Application
Antibody used in WB on Human MCF7 at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Liver.Application
Antibody used in WB on Mouse heart at: 1:1000 (Lane 1: 40ug mouse heart lysate, Lane 2: 40ug mouse heart lysate).| Product Name | GCLM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GCLM, GLCLR |
| Product description | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Jonsson, L.S., (2008) Int Arch Occup Environ Health 81 (7), 913-919. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GCLM. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GCLM antibody can be used for detection of GCLM by ELISA at 1:312500. GCLM antibody can be used for detection of GCLM by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GCLM |
|---|---|
| Alternative Names | GCLM GLCLR |
| Molecular Weight(MW) | 31 kDa |
Application
Antibody used in WB on Human MCF7 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Liver.
Application
Antibody used in WB on Mouse heart at: 1:1000 (Lane 1: 40ug mouse heart lysate, Lane 2: 40ug mouse heart lysate).| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | GCLM antibody can be used for detection of GCLM by ELISA at 1:312500. GCLM antibody can be used for detection of GCLM by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GCLM antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
