Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: U-87 MG whole cell lysate Lane 3: Hela whole cell lysate Lane 4: rat brain lysate Lane 5: mouse brain lysate Lane 6: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: H. brain whole lysate Lane 4: M. brain whole lysate Lane 5: R. brain whole lysate Lysates/proteins at 20 ug per lane.| Product Name | GDI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rab GDP dissociation inhibitor alpha, Rab GDI alpha, Guanosine diphosphate dissociation inhibitor 1, GDI-1, Oligophrenin-2, Protein XAP-4, GDI1, GDIL, OPHN2, RABGDIA, XAP4 |
| Product description | GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation.1) Martins-de-Souza, D., et al.Psychiatr Res 44(14):989-991(2010) |
| Immunogen | This GDI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-443 amino acids from the C-terminal region of human GDI1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000-1:2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GDI1 |
|---|---|
| Alternative Names | Rab GDP dissociation inhibitor alpha Rab GDI alpha Guanosine diphosphate dissociation inhibitor 1 GDI-1 Oligophrenin-2 Protein XAP-4 GDI1 GDIL OPHN2 RABGDIA XAP4 |
| Molecular Weight(MW) | 51 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: U-87 MG whole cell lysate Lane 3: Hela whole cell lysate Lane 4: rat brain lysate Lane 5: mouse brain lysate Lane 6: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: H. brain whole lysate Lane 4: M. brain whole lysate Lane 5: R. brain whole lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000-1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
