Application
Antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Germinal Center Kinase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase kinase kinase kinase 2, B lymphocyte serine/threonine-protein kinase, Germinal center kinase, GC kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, MEKKK 2, Rab8-interacting protein, MAP4K2, GCK, RAB8IP |
| Product description | The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.1) Chadee, D.N., et al., Mol. Cell. Biol. 22(3):737-749 (2002). |
| Immunogen | This Germinal Center Kinase (GCK/MAP4K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-319 amino acids from the Central region of human Germinal Center Kinase (GCK/MAP4K2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | MAP4K2 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase kinase kinase kinase 2 B lymphocyte serine/threonine-protein kinase Germinal center kinase GC kinase MAPK/ERK kinase kinase kinase 2 MEK kinase kinase 2 MEKKK 2 Rab8-interacting protein MAP4K2 GCK RAB8IP |
| Molecular Weight(MW) | 92 kDa |
Application
Antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
