Application
Western blot analysis of GFAP using rabbit polyclonal GFAP Antibody (T396) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GFAP gene.Application
Antibody is used in Western blot to detect GFAP in mouse brain tissue lysate.| Product Name | GFAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glial fibrillary acidic protein, GFAP, GFAP |
| Product description | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system.1) Quintanar, J.L., et al., Parasitol. Res. 90(4):261-263 (2003). |
| Immunogen | This GFAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-410 amino acids from the C-terminal region of human GFAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | GFAP |
|---|---|
| Alternative Names | Glial fibrillary acidic protein GFAP GFAP |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of GFAP using rabbit polyclonal GFAP Antibody (T396) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GFAP gene.
Application
Antibody is used in Western blot to detect GFAP in mouse brain tissue lysate.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
