Application
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
GFPT2 Antibody IHC analysis in formalin fixed and paraffin embedded human skin followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of GFPT2 Antibody in A2058, K562 cell line lysates (35ug/lane)| Product Name | GFPT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GFPT2 controls the flux of glucose into the hexosamine pathway. This protein most likely involved in regulating the availability of precursors for N-and O-linked glycosylation of proteins.1) Edwards,T.L., et.al., Ann. Hum. Genet. 74 (2), 97-109 (2010) |
| Immunogen | This GFPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-201 amino acids from the Central region of human GFPT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GFPT2 |
|---|---|
| Alternative Names | Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 D-fructose-6-phosphate amidotransferase 2 Glutamine:fructose-6-phosphate amidotransferase 2 GFAT 2 GFAT2 Hexosephosphate aminotransferase 2 GFPT2 |
| Molecular Weight(MW) | 77 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
GFPT2 Antibody IHC analysis in formalin fixed and paraffin embedded human skin followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of GFPT2 Antibody in A2058, K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
