Application
Antibody used in WB on Rat 0.25 ug/ml.Application
Antibody used in WB on rat stiatum at 1:1000.Application
Antibody used in WB on Rat cardiac at 0.25 ug/ml (Lane1: 8 ug total cardiac lysate, Lane2: 15 ug total cardiac lysate, Lane3: 30 ug total cardiac lysate , Lane4: 50 ug total cardiac lysate).Application
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.| Product Name | GJA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GJA1, HSS, CMDR, CX43, GJAL, ODDD, AVSD3, HLHS1, DFNB38 |
| Product description | Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.1) Gellhaus, A., et al., (2004) J. Biol. Chem. 279 (35), 36931-36942. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GJA1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GJA1 antibody can be used for detection of GJA1 by ELISA at 1:312500. GJA1 antibody can be used for detection of GJA1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GJA1 |
|---|---|
| Alternative Names | GJA1 HSS CMDR CX43 GJAL ODDD AVSD3 HLHS1 DFNB38 |
| Molecular Weight(MW) | 42 kDa |
Application
Antibody used in WB on Rat 0.25 ug/ml.
Application
Antibody used in WB on rat stiatum at 1:1000.
Application
Antibody used in WB on Rat cardiac at 0.25 ug/ml (Lane1: 8 ug total cardiac lysate, Lane2: 15 ug total cardiac lysate, Lane3: 30 ug total cardiac lysate , Lane4: 50 ug total cardiac lysate).
Application
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1309 - Human Placenta Lysate |
|---|---|
| Application Notes | GJA1 antibody can be used for detection of GJA1 by ELISA at 1:312500. GJA1 antibody can be used for detection of GJA1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GJA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
