Application
Western blot analysis of GJB2 in human colon tissue lysate with GJB2 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | GJB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gap junction beta-2 protein, connexin 26, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, HID, KID, NSRD1, PPK |
| Product description | The Gap junction beta-2 protein (GJB2), also known as Connexin 26, is member of the gap junction protein family which form structures that were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells (1). Mutations in the GJB2 gene are thought to be responsible for as much as 35-45% of congenital sensorineural hearing loss in some populations (2). Other mutations in this gene have also been linked to a wide array of skin diseases (3).1) Zhou JZ and Jiang JX. Gap junctions and hemichannel-independent actions of connexins on cell and tissue functions – An update. FEBS Lett. 2014; 588:1186-92. |
| Immunogen | GJB2 antibody was raised against a 16 amino acid peptide near the center of human GJB2. The immunogen is located within amino acids 80 - 130 of GJB2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| GJB2 antibody can be used for detection of GJB2 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GJB2 |
|---|---|
| Alternative Names | Gap junction beta-2 protein connexin 26 CX26 DFNA3 DFNA3A DFNB1 DFNB1A HID KID NSRD1 PPK |
| Molecular Weight(MW) | Predicted: 25 kDa Observed: 26 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (94%) |
| Tissue Specificity | GJB2 antibody is human specific. |
Application
Western blot analysis of GJB2 in human colon tissue lysate with GJB2 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | GJB2 antibody can be used for detection of GJB2 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GJB2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | GJB2 antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM279814