Application
Antibody used in WB on Lane 1: 4ug mCx26 elution fraction 6 Lane 2: 4ug mCx26 elution fraction 7Lane 3: 4ug mCx26 elution fraction 6 + other Cx26 antibody Lane 4: 4ug mCx26 elution fraction 7 + other Cx26 antibody at 1:3000 (Lane 1: 4ug mCx26 elution fraction 6 Lane 2: 4ug mCx26 elution fraction 7Lane 3: 4ug mCx26 elution fraction 6 + other Cx26 antibody Lane 4: 4ug mCx26 elution fraction 7 + otheApplication
Antibody used in WB on Human Lung at 1.25 ug/ml.| Product Name | GJB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 (MIM 121014) is designated alpha-1 gap junction protein, whereas CX32 (GJB1; MIM 304040) and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43.1) Hromas, R., et al., (2004) Neurosci. Res. 50 (1), 125-128. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GJB2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,WB |
| GJB2 antibody can be used for detection of GJB2 by ELISA at 1:62500. GJB2 antibody can be used for detection of GJB2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Dog,Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GJB2 |
|---|---|
| Alternative Names | GJB2 HID KID PPK CX26 DFNA3 DFNB1 NSRD1 DFNA3A DFNB1A |
| Molecular Weight(MW) | 25 kDa |
Application
Antibody used in WB on Lane 1: 4ug mCx26 elution fraction 6 Lane 2: 4ug mCx26 elution fraction 7Lane 3: 4ug mCx26 elution fraction 6 + other Cx26 antibody Lane 4: 4ug mCx26 elution fraction 7 + other Cx26 antibody at 1:3000 (Lane 1: 4ug mCx26 elution fraction 6 Lane 2: 4ug mCx26 elution fraction 7Lane 3: 4ug mCx26 elution fraction 6 + other Cx26 antibody Lane 4: 4ug mCx26 elution fraction 7 + othe
Application
Antibody used in WB on Human Lung at 1.25 ug/ml.| Application Notes | GJB2 antibody can be used for detection of GJB2 by ELISA at 1:62500. GJB2 antibody can be used for detection of GJB2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GJB2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
Data sheet for OM279815