Application
Immunohistochemistry of GLIS2 in mouse kidney tissue with GLIS2 antibody at 5 μg/ml.Application
Western blot analysis of GLIS2 in K562 cell lysate with GLIS2 antibody at 1 μg/ml.| Product Name | GLIS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLIS family zinc finger 2, GLI-similar 2, neuronal krueppel-like protein, NKL, NPHP7 |
| Product description | GLIS2, also known as neuronal Krueppel-like, is a 524 amino acid protein that belongs to the GLI C2H2-type zinc-finger protein family (1). GLIS2 can act either as a transcription repressor or as a transcription activator and may be involved in neuron differentiation (1,2). GLIS2 is expressed at high levels in kidney and at low levels in heart, lung and placenta. Mutations of GLIS2 have been suggested to be associated with development of progressive chronic kidney disease with characteristics resembling nephronophthisis (3,4).1) Vasanth S, ZeRuth G, Kang HS, et al. Identification of nuclear localization, DNA binding, and transactivating mechanisms of Kruppel-like zinc finger protein Gli-similar 2 (Glis2). J. Biol. Chem. 2011; 286:4749-59. |
| Immunogen | GLIS2 antibody was raised against an 18 amino acid peptide near the center of human GLIS2. The immunogen is located within amino acids 260 - 310 of GLIS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| GLIS2 antibody can be used for detection of GLIS2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GLIS2 |
|---|---|
| Alternative Names | GLIS family zinc finger 2 GLI-similar 2 neuronal krueppel-like protein NKL NPHP7 |
| Molecular Weight(MW) | Predicted: 58 kDa Observed: 60 kDa |
| Tissue Specificity | GLIS2 antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of GLIS2 in mouse kidney tissue with GLIS2 antibody at 5 μg/ml.
Application
Western blot analysis of GLIS2 in K562 cell lysate with GLIS2 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | GLIS2 antibody can be used for detection of GLIS2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GLIS2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | GLIS2 antibody is supplied in PBS containing 0.02% sodium azide. |
