Application
Antibody used in IHC on Human Pancreas at 1:10.Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Product Name | GLIS3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLIS3, ZNF515 |
| Product description | GLIS3 is a member of the GLI-similar zinc finger protein family and has five C2H2-type zinc finger domains. GLIS3 functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH).1) Kim, Y.S., (2003) Nucleic Acids Res. 31 (19), 5513-5525. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GLIS3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GLIS3 antibody can be used for detection of GLIS3 by ELISA at 1:62500. GLIS3 antibody can be used for detection of GLIS3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GLIS3 |
|---|---|
| Alternative Names | GLIS3 ZNF515 |
| Molecular Weight(MW) | 84 kDa, 100 kDa |
Application
Antibody used in IHC on Human Pancreas at 1:10.
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | GLIS3 antibody can be used for detection of GLIS3 by ELISA at 1:62500. GLIS3 antibody can be used for detection of GLIS3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GLIS3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
