Application
Western blot analysis of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody at 1:1000 at each lane.Application
Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).Application
Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).Application
Immunohistochemical analysis of paraffin-embedded H.kidney section using GLS Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: human kidney lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | GLS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838 |
| Product description | Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.1) Swierczynski,J., et.al., Biochim. Biophys. Acta 1157 (1), 55-62 (1993) |
| Immunogen | This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:25 For IF starting dilution is: 1:25 For IF starting dilution is: 1:25: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GLS |
|---|---|
| Alternative Names | Glutaminase kidney isoform mitochondrial GLS K-glutaminase L-glutamine amidohydrolase GLS GLS1 KIAA0838 |
| Molecular Weight(MW) | 73 kDa |
Application
Western blot analysis of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody at 1:1000 at each lane.
Application
Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Application
Fluorescent image of Hela cells stained with XAF1 GLS Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Application
Immunohistochemical analysis of paraffin-embedded H.kidney section using GLS Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: human kidney lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:25 For IF starting dilution is: 1:25 For IF starting dilution is: 1:25: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
