Application
Immunofluorescence of GLS2 in mouse brain tissue with GLS2 Antibodyat 20 μg/mL.Application
Immunohistochemistry of GLS2 in rat liver tissue with GLS2 antibody at 5 μg/mL.Application
Immunohistochemistry of GLS2 in mouse brain tissue with GLS2 Antibodyat 5 μg/mL.Application
Immunofluorescence of GLS2 in rat liver cells with GLS2 antibody at 20 5 μg/mL.Application
Western blot analysis of GLS2 in rat liver tissue lysate with GLS2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | GLS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLS2 Antibody: GA, GLS, LGA, hLGA, GA, Glutaminase liver isoform, mitochondrial, L-glutaminase |
| Product description | GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.1) Chung-Bok MI, Vincent N, Jhala U, et al. Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter. Biochem. J. 1997; 324:193-200. |
| Immunogen | GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2. The immunogen is located within amino acids 300 - 350 of GLS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GLS2 |
|---|---|
| Alternative Names | GLS2 Antibody: GA GLS LGA hLGA GA Glutaminase liver isoform mitochondrial L-glutaminase |
| Molecular Weight(MW) | Predicted: 36, 55, 62, 66 kDa Observed: 55, 62 kDa |
| Tissue Specificity | Multiple isoforms of GLS2 are known to exist. |
Application
Immunofluorescence of GLS2 in mouse brain tissue with GLS2 Antibodyat 20 μg/mL.
Application
Immunohistochemistry of GLS2 in rat liver tissue with GLS2 antibody at 5 μg/mL.
Application
Immunohistochemistry of GLS2 in mouse brain tissue with GLS2 Antibodyat 5 μg/mL.
Application
Immunofluorescence of GLS2 in rat liver cells with GLS2 antibody at 20 5 μg/mL.
Application
Western blot analysis of GLS2 in rat liver tissue lysate with GLS2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GLS2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GLS2 Antibody is supplied in PBS containing 0.02% sodium azide. |
