Application
Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845.| Product Name | GluR1 (phospho Ser845) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GluA1, gluR-A, Glur1, AMPA-selective glutamate receptor 1, GluR-1 |
| Product description | The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of 4 distinct subunits (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser845. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.1) Roche, K.W., O'Brien, R.J., Mammen, A.L., Bernhardt, J., and Huganir, R.L., "Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit," Neuron, 16 (1996) 1179 - 1188. |
| Immunogen | GluR1 (Ser845) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser845 of rat GluR1. |
| Modification | p-Ser845 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Immunolabeling of the GluR1 protein in western blots of rat brain is blocked by the Ser845 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Gria1 |
|---|---|
| Alternative Names | GluA1 gluR-A Glur1 AMPA-selective glutamate receptor 1 GluR-1 |
| Molecular Weight(MW) | 100 |
| Tissue Specificity | GluR1 antibody is specific for GluR1 phosphorylated at Ser845. |
Application
Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845.| Application Notes | Immunolabeling of the GluR1 protein in western blots of rat brain is blocked by the Ser845 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
