Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Immunofluorecence staining of anti-GPC3 Pab on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1:50. Original magnification 1:400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.| Product Name | Glypican 3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5 |
| Product description | GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.1) Nakatsura, T., et al., Clin. Cancer Res. 10(19):6612-6621 (2004). |
| Immunogen | This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-365 amino acids from the Central region of human Glypican 3 (GPC3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: Tested: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | GPC3 |
|---|---|
| Alternative Names | Glypican-3 GTR2-2 Intestinal protein OCI-5 MXR7 Secreted glypican-3 GPC3 OCI5 |
| Molecular Weight(MW) | 66 kDa |
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Immunofluorecence staining of anti-GPC3 Pab on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1:50. Original magnification 1:400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: Tested: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
