Application
Antibody used in WB on Human MCF7 cells at 1 ug/ml.Application
Antibody used in WB on INS1 at: 1:1000.Application
Antibody used in IHC on Human Pancreas at 5 ug/ml.Application
Antibody used in IHC on Human thyroid at 5 ug/ml.| Product Name | GNAS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GNAS, AHO, C20orf45, GNAS1, GPSA, GSA, GSP, MGC33735, PHP1A, PHP1B, POH, dJ309F20.1.1, dJ806M20.3.3, NESP, PHP1C |
| Product description | Mutations in GNAS gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript exists, and this antisense transcript and one of the transcripts are paternally expressed, produce noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.1) Bagchi, G., (2008) Cancer Res. 68 (9), 3225-3231. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GNAS. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GNAS antibody can be used for detection of GNAS by ELISA at 1:62500. GNAS antibody can be used for detection of GNAS by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GNAS |
|---|---|
| Alternative Names | GNAS AHO C20orf45 GNAS1 GPSA GSA GSP MGC33735 PHP1A PHP1B POH dJ309F20.1.1 dJ806M20.3.3 NESP PHP1C |
| Molecular Weight(MW) | 46 kDa |
Application
Antibody used in WB on Human MCF7 cells at 1 ug/ml.
Application
Antibody used in WB on INS1 at: 1:1000.
Application
Antibody used in IHC on Human Pancreas at 5 ug/ml.
Application
Antibody used in IHC on Human thyroid at 5 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | GNAS antibody can be used for detection of GNAS by ELISA at 1:62500. GNAS antibody can be used for detection of GNAS by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GNAS antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
