Application
Immunohistochemistry staining of GNAS in thyroid tissue using GNAS Antibody.| Product Name | GNAS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GNAS, AHO, C20orf45, Extra large alphas protein, GSP, GNAS complex locus, GNASXL, NESP, PHP1B, PHP1C, PHP1A, POH, SCG6, XLalphas, GNAS1, GPSA, GSA, NESP55, Protein ALEX, Secretogranin VI |
| Product description | The GNAS1 gene encodes the alpha subunit of the G protein Gs, which couples receptor binding by several hormones to activation of adenylate cyclase (Hayward et al., 1998). This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript exists, and this antisense transcript and one of the transcripts are paternally expressed, produce noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. |
| Immunogen | GNAS antibody was raised against amino acids 385 - 394 of GNAS (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC |
| GNAS antibody can be used in ELISA starting at 1:5000 - 1:50000, Western Blot starting at 1:5000 - 1:50000, immunohistochemistry starting at 2.5 μg/mL, and immunoprecipitation: |
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| Species Reactivity | HumanPrimate |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | GNAS |
|---|---|
| Alternative Names | GNAS AHO C20orf45 Extra large alphas protein GSP GNAS complex locus GNASXL NESP PHP1B PHP1C PHP1A POH SCG6 XLalphas GNAS1 GPSA GSA NESP55 Protein ALEX Secretogranin VI |
Application
Immunohistochemistry staining of GNAS in thyroid tissue using GNAS Antibody.| Application Notes | GNAS antibody can be used in ELISA starting at 1:5000 - 1:50000, Western Blot starting at 1:5000 - 1:50000, immunohistochemistry starting at 2.5 μg/mL, and immunoprecipitation: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GNAS antibody should be stored long term (months) at -20 ˚C and short term (weeks) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.1% sodium azide. |
