Application
Immunocytochemistry of GNPDA1 in 293 cells with GNPDA1 antibody at 5 μg/mL.Application
Immunofluorescence of GNPDA1 in 293 cells with GNPDA1 antibody at 20 μg/mL.Application
Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | GNPDA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GNPDA1 Antibody: GPI, HLN, GNP1, GNPI, GNPDA, KIAA0060, Glucosamine-6-phosphate isomerase 1, Glucosamine-6-phosphate deaminase 1, GNPDA 1 |
| Product description | GNPDA1 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate deaminases that are thought to have arisen though gene duplication, with the GNPDA2 protein closer in structure and activity to the E. coli enzyme. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.1) Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J.1998; 12:91-9. |
| Immunogen | GNPDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GNPDA1. The immunogen is located within the last 50 amino acids of GNPDA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GNPDA1 |
|---|---|
| Alternative Names | GNPDA1 Antibody: GPI HLN GNP1 GNPI GNPDA KIAA0060 Glucosamine-6-phosphate isomerase 1 Glucosamine-6-phosphate deaminase 1 GNPDA 1 |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | GNPDA1 is predicted to not cross-react with GNPDA2. |
Application
Immunocytochemistry of GNPDA1 in 293 cells with GNPDA1 antibody at 5 μg/mL.
Application
Immunofluorescence of GNPDA1 in 293 cells with GNPDA1 antibody at 20 μg/mL.
Application
Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GNPDA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GNPDA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
