Application
Immunohistochemistry of GOLPH2 in rat brain tissue with GOLPH2 antibody at 2.5 μg/mL.Application
Immunofluorescence of GOLPH2 in rat brain tissue with GOLPH2 antibody at 20 μg/mL.Application
Western blot analysis of GOLPH2 in rat brain tissue lysate with GOLPH2 antibody at (A) 0.25 and (B) 0.5 μg/mL.| Product Name | GOLPH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GOLPH2 Antibody: GP73, HEL46, GOLPH2, C9orf155, PSEC0257, bA379P1.3, PSEC0242, UNQ686/PRO1326, Golgi membrane protein 1, Golgi membrane protein GP73 |
| Product description | GOLPH2 Antibody: GOLPH2, also known as GOLM1, is a Golgi phosphoprotein that has a short cytoplasmic N-terminal domain, a membrane spanning region, and a longer C-terminal domain. It was initially identified as a possible marker for Alzheimer's disease, although later studies have demonstrated that the GOLPH2 gene does not contribute to risk of this disease. GOLPH2 expression has been reported higher in prostate cancer tissues compared to normal prostate tissue, suggesting that GOLPH2 can be used as an additional positive marker for tissue-based diagnosis of prostate cancer. It has been suggested that GOLPH2 expression in hepatocellular carcinomas (HCCs) and serum may also serve as tumor markers for HCCs.1) Kladney RD, Bulla GA, Guo L, et al. GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 2000; 249:53-65. |
| Immunogen | GOLPH2 antibody was raised against an 18 amino acid synthetic peptide from near the center of human GOLPH2. The immunogen is located within amino acids 130 - 180 of GOLPH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GOLPH2 antibody can be used for detection of GOLPH2 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GOLM1 |
|---|---|
| Alternative Names | GOLPH2 Antibody: GP73 HEL46 GOLPH2 C9orf155 PSEC0257 bA379P1.3 PSEC0242 UNQ686/PRO1326 Golgi membrane protein 1 Golgi membrane protein GP73 |
| Molecular Weight(MW) | Predicted: 67 kDa Observed: 70 kDa |
Application
Immunohistochemistry of GOLPH2 in rat brain tissue with GOLPH2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of GOLPH2 in rat brain tissue with GOLPH2 antibody at 20 μg/mL.
Application
Western blot analysis of GOLPH2 in rat brain tissue lysate with GOLPH2 antibody at (A) 0.25 and (B) 0.5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | GOLPH2 antibody can be used for detection of GOLPH2 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GOLPH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GOLPH2 Antibody is supplied in PBS containing 0.02% sodium azide. |
