Application
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
GOT1 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of GOT1 Antibody in mouse liver tissue lysates (35ug/lane)Application
Western blot analysis of GOT1 using rabbit polyclonal GOT1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GOT1 gene.| Product Name | GOT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aspartate aminotransferase, cytoplasmic, cAspAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, cCAT, Glutamate oxaloacetate transaminase 1, Transaminase A, GOT1 |
| Product description | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.1) Franke, A., et al. Nat. Genet. 42(4):292-294(2010) |
| Immunogen | This GOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-381 amino acids from the C-terminal region of human GOT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GOT1 |
|---|---|
| Alternative Names | Aspartate aminotransferase cytoplasmic cAspAT Cysteine aminotransferase cytoplasmic Cysteine transaminase cytoplasmic cCAT Glutamate oxaloacetate transaminase 1 Transaminase A GOT1 |
| Molecular Weight(MW) | 46 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
GOT1 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of GOT1 Antibody in mouse liver tissue lysates (35ug/lane)
Application
Western blot analysis of GOT1 using rabbit polyclonal GOT1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GOT1 gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
